Tuesday, May 14, 2013

Microscope

To focus on an item using a microscope you always start with the scanning lens. When you focus on that lens you can move up to low power and then high power. This will ensure that you get an accurate view of the object and it will keep you from trying to focus on high power for a long time. 

To make a wet mount slide you first get your slide and add one drop of water using a pipet. Then you place an object on the slide. Next you carefully lower the cover slip onto the slide (try to avoid air bubbles for a more accurate view). Lastly you absorb the excess water with a paper towel and you're ready to view.

To stain a slide you prepare a wet mount slide (as explained above) then you use the pipet to get a few drops of stain. Next you place a single drop of stain on the outer edge of the cover slip. After that you take a paper towel and set it against the opposite side of the cover slip, this will pull the water out and suck the stain under the cover slip. If the stain does not come all the way through, add another drop of stain to the cover slip.

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